FastQCFastQC Report
Fri 10 Feb 2017
SRR3559099.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559099.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3534793
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT846382.394425925365361No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT406371.1496288467245466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272470.770823072242137No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164330.4648928522830049No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155910.44107250410420074No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA109860.3107961343139471No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103900.293935175270518No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT80520.22779268828471708No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66090.18696993006379722No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65680.18581003187456804No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG55630.15737838113858435No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC49860.14105493589016388No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA39820.11265157535391747No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38670.1093982023841283No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37280.10546586462064399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG5550.064.3684542
TACAACG7700.055.6745342
TAACGCA8250.050.519494
CGCATAG8600.047.5323451
GTATCAA418000.044.8988651
ACAACGC9850.043.5222243
GTATCAC11900.039.90791
TCACGCA8950.039.2503744
ATCACGC9650.037.6372033
GTATAAC10300.037.352661
GTACAAC12300.037.143881
TATCAAC511250.036.4290852
AACGCAG544850.036.0975846
TCAACGC527750.036.0121844
ATCAACG516800.035.9226573
CAACGCA533050.035.903385
AGATTAC16350.035.688082
TAGTACT12350.035.16485
ACGCAGA594400.033.078437
ATAACGC14650.032.920183
Produced by FastQC (version 0.11.5)