FastQCFastQC Report
Fri 10 Feb 2017
SRR3559079.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559079.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5978080
Sequences flagged as poor quality0
Sequence length125
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91092315.237718464791373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4435187.41907100607553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2365653.9572069962262133No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1632582.730943714369831No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1123521.8793994058292962No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT762921.2761957016299548No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT688911.1523934105933678No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT649781.086937612076118No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381050.6374120118834141No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276580.46265690656531866No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234190.3917478521531997No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA140620.23522602574739715No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA139470.23330233118325616No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133120.22268019163343414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC131510.21998701924363676No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121650.20349342932848005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC91660.1533268206514466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT83590.13982750314482242No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCT79940.13372186387602708No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT79780.13345421941492921No Hit
GCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAGTGAGCAAGGGCGAGGA77310.12932245804673073No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA75390.12611072451355618No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATA74330.12433757995878275No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70040.1171613628455959No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACA67550.11299614591976019No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65470.10951676792548778No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATACA64590.10804472338944945No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63770.10667304552632283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA62100.10387950646361373No Hit
GTATCAACGCAGAGTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGT60350.1009521451703557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2096050.080.920421
GGTATCA677800.068.9158551
TTGGACT8650.068.178454
TTTGGAC9250.065.044073
GACCGTT4750.062.588897
TTAGGAC5000.061.952863
TATCAAC2758150.061.6099782
TAGGACC5050.060.1598634
CGCACAG3400.059.4619268
ATCAACG2910400.058.3890533
TGGACTG10500.058.323475
TCAACGC2957350.057.528564
CAACGCA3010100.056.522775
AACGCAG3015400.056.3379946
GCGTATC3900.055.286581
CTTAGGA5500.055.237692
GCCTAGT6300.055.140321
AGTATCA24850.051.3375321
ACGCAGA3375600.050.3333857
CGCAGAG3396950.050.024388
Produced by FastQC (version 0.11.5)