FastQCFastQC Report
Fri 10 Feb 2017
SRR3559067.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559067.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31249031
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4405281.4097333130105698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2002520.6408262707410031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1057160.33830169005880534No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT756910.24221871071778192No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469670.15029906047326716No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453740.1452013024019849No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388360.12427905364489542No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC333190.10662410620028506No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314350.10059511925345781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1568800.071.9091641
TATCAAC2137650.052.607122
ATCAACG2161900.051.8127333
TCAACGC2211600.051.227174
CAACGCA2225750.050.9468155
AACGCAG2319300.049.4279676
ACGCAGA2595950.044.0343867
CGCAGAG2662700.042.9640248
GGTATCA622900.042.0408131
GCAGAGT2812600.040.65739
GTACATG2035650.032.0038071
ACATGGG1994200.031.5212863
TACATGG2062400.031.44922
AGAGTAC2735050.031.26968610-11
CATGGGG965050.030.020184
CATGGGT395950.029.5469844
AGTACTT1791000.028.64820312-13
GTACTGG214950.026.7274461
GAGTACT1626550.026.06497612-13
TACTGGT188250.025.8710142
Produced by FastQC (version 0.11.5)