FastQCFastQC Report
Fri 10 Feb 2017
SRR3559065.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559065.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119296
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45543.8173953862660945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22261.8659468884120172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12351.035240075107296No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8510.7133516630901288No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7000.5867757510729613No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.4283462982832618No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.3386534334763948No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.307638143776824No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2970.2489605686695279No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2470.20704801502145925No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC2130.17854747854077255No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT2000.1676502145922747No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1930.16178245708154507No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA1660.139149678111588No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.13076716738197425No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC1460.12238465665236052No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.12070815450643778No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT1400.11735515021459228No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG1380.11567864806866952No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAC250.00200948171.780431
ACAACGC250.002043316671.4790953
TACTGGT603.8926373E-969.493572
GTACTGG954.0017767E-1156.668771
GTATCAA16350.053.0487441
TCACGCA350.007745234851.0564964
TATCACG350.007745234851.0564962
ATCGTCT350.00778389250.992278
GTAGCCA350.00778389250.992279
AGATTAC505.28433E-447.652732
GATTACT505.28433E-447.652733
AGTACTC505.28433E-447.652733
GTATAAA909.231371E-846.524361
GGTTCAC951.4743091E-743.8354646
TATCAAC20400.042.3385122
ACTGGTT1002.1841151E-741.696143
TCACTAT1002.2058339E-741.643699
ATCAACG21300.040.5495643
AACGCAG22850.040.354946
TCAACGC21800.040.1660044
Produced by FastQC (version 0.11.5)