FastQCFastQC Report
Fri 10 Feb 2017
SRR3559060.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559060.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182608
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65103.565013581004118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33191.817554543064926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19291.0563611670901605No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.7507885744326646No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.5569306930693069No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7180.39319197406466305No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.3017392447209323No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.3017392447209323No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3620.19823885043371595No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3530.19331026022956277No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC3420.18728642775782003No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT3020.1653815824060282No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA2650.14511960045562078No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC2350.1286909664417769No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.12540523963900815No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2210.12102427056864978No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.11554805923070183No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG2090.11445281696311224No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT1860.10185753088583194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC358.888055E-785.06423
TATCACG359.097767E-568.051362
ATAGTAC701.72804E-1068.051364
CGCATAG753.0559022E-1063.8118931
TAACGCA401.7614254E-459.5449454
GACCGTT300.00423197359.496037
GGGTACC300.00423197359.496037
GTATCAA22400.058.48831
TAGGACC954.1836756E-1156.4109954
GCAAAGT752.2846507E-855.575282
TCACGCA453.1520796E-452.9288374
GATTACT952.5956979E-950.143113
ATAGGAC505.300972E-447.6359523
GCATAGT1004.08545E-947.6359522
AGATTAC1056.2918843E-945.3675772
AGGACCG1056.337359E-945.3303075
GGGCTAT802.2842978E-644.622026
TAGTACT1204.1472958E-1044.622025
GTGTACC558.285147E-443.508111
TATCAAC30150.043.4490472
Produced by FastQC (version 0.11.5)