FastQCFastQC Report
Fri 10 Feb 2017
SRR3559006.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559006.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376018
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190585.068374386332569No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94702.5184964549569435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83632.2240956549952395No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33260.8845321234621747No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17770.47258375928811924No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16580.4409363381540245No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.4138099771819434No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14100.37498204873170965No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11500.30583642272444406No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11330.3013153625624305No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10140.2696679414283359No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9900.2632852682584344No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT9040.24041402273295426No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.23456323899387793No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.23057406826268956No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC8020.21328766176087316No Hit
GTACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.1944055869665814No Hit
GTATAAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.19015047151998044No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT6920.18403374306549153No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.18350185363466642No Hit
GTGTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.1816402406267785No Hit
CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6700.17818295932641523No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA6190.16461977884037465No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC6160.16382194469413697No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT6070.16142844225542394No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA6040.16063060810918628No Hit
GTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5930.15770521623964812No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5840.15531171380093506No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5740.15265226664680948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5630.1497268747772713No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.14467392518443267No Hit
GTATTAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.1404188097378317No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC5190.13802530729911866No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT5170.13749341786829353No Hit
CTGCACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT5030.1337701918525177RNA PCR Primer, Index 46 (95% over 21bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG4970.13217452356004233No Hit
CAGATTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.1316426341292172No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA4890.13004696583674186No Hit
GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.1284512975442665No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.12685562925179114No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC4740.12605779510555346No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA4390.11674973006611386No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT4150.11036705689621241No Hit
TATCTACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.11010111218079986No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.11010111218079986No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA4130.1098351674653873No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC4100.10903733331914961No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT4100.10903733331914961No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4090.10877138860373706No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4030.1071757203112617No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3960.10531410730337377No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC3950.10504816258796121No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC3910.10398438372631097No Hit
GTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.10371843901089842No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC3880.1031865495800733No Hit
GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACTAAGG3840.10212277071842306No Hit
GCACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT3800.1010589918567728RNA PCR Primer, Index 46 (95% over 23bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA3800.1010589918567728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAC1750.092.678631
CGCATAG3550.091.37331
GTATAAC2000.087.1007461
TACAACG1800.085.9591452
ATAACGC2100.085.0145343
GAGTTCT3450.080.092641
ATAGTAC4150.078.868914
AGATTAC6250.078.0773542
GTATCAC2100.077.232191
GGTTTCA3450.076.610351
TCACGCA1800.076.040784
GCATAGT4300.074.733712
CATAGTA4550.071.935383
AGTTCTT3750.071.412222
TAGTACT4700.070.886895
TATCACG1950.070.191492
ACAACGC2300.069.859773
TAAACGC1600.066.9489444
ATCATCG657.4433046E-964.087883
CAACTCA1050.062.929931
Produced by FastQC (version 0.11.5)