FastQCFastQC Report
Fri 10 Feb 2017
SRR3558962.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558962.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596400
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT617861.7179957735513292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292600.8135913691469246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218360.6071627182738294No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA160260.4456122789456123No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT121330.33736514292069847No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT113760.3163163163163163No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94810.26362473584695806No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC85190.2368757646535424No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG84640.23534645756867978No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT81010.22525303080858636No Hit
GTAATAAACACCCACATCCTCAGCCTCCACTCTACTGATTCTCAGTGTGA70550.19616839061283503No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG70200.19519519519519518No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA68660.1909131353575798No Hit
TTATTACTGTATGCAACATCTAGAATATCCTTTCACGTTCGGCTCGGGGA67860.18868868868868868No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC67330.18721499277054832No Hit
CAGTAATAAACACCCACATCCTCAGCCTCCACTCTACTGATTCTCAGTGT60430.16802914025136245No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG58430.16246802357913467No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT57670.16035479924368812No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG57500.15988210432654879No Hit
GTTTATTACTGTATGCAACATCTAGAATATCCTTTCACGTTCGGCTCGGG55690.15484929373818263No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55120.1532643754865977No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA54470.15145701256812366No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT54110.15045601156712268No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT51300.14264264264264265No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT50900.14153041930819707No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT50680.14091869647425204No Hit
CTCCTGGAGAGTCAGTATCCATCTCCTGCAGGTCTAGTAAGAGTCTCCTG49840.13858302747191636No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT48990.13621955288621954No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT46200.1284617951284618No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45920.1276832387943499No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC45500.1265154042931821No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA45430.1263207652096541No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC44920.12490268045823602No Hit
CTCTGTACCTGTCACTCCTGGAGAGTCAGTATCCATCTCCTGCAGGTCTA44750.12442998554109666No Hit
TGGTTATACTGAGTACAATGAGAAGTTCAAGGGCAAGGCCACACTGACTT43550.12109331553775998No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG42330.11770103436770103No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA40580.11283505727950173No Hit
CAGTTGGACTGATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCA40320.11211211211211211No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC39630.11019352686019353No Hit
TTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACT39210.10902569235902569No Hit
GGCCAGGCCAGTCTCCTCAGCTCCTGATATATCGGATGTCCAACCTTGCC39090.10869202535869202No Hit
ACATAACAGCTATACCTGTGAGGCCACTCACAAGACATCAACTTCACCCA39030.10852519185852519No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT38440.10688466244021799No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC38350.10663441218996775No Hit
TCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTCAGTCGTG37760.10499388277166055No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG37670.1047436325214103No Hit
GTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACAT37530.10435435435435436No Hit
GTATCCATCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTC37360.10388165943721499No Hit
TGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACG37350.10385385385385384No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT37340.1038260482704927No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36750.10218551885218552No Hit
GTCTGGGACTCCTGAGGCAAGGTTGGACATCCGATATATCAGGAGCTGAG36510.10151818485151819No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA36180.10060060060060061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG3450.067.252082
TCACGCA4000.065.4414444
TATCACG4450.058.8237652
GTATCAC6050.049.4324041
CGCATAG4450.048.3882141
TAACGCA4150.045.8735244
AGATTAC7850.043.9560362
GTACAAC5750.043.689651
ATCACGC6100.042.9124223
GACCGTT3050.040.9612857
ACAACGC6750.037.0173843
AAACGCA6700.036.405185
AGTGTAC9650.036.373483
TACTGGT14550.035.1637882
ATAGTAC5850.034.5766754
GATTACT10300.034.0780683
GTACTGG17900.033.081051
CATGGGG72000.032.7207224
ATAACGC7700.031.6776773
TGCAAAG15750.031.3514562
Produced by FastQC (version 0.11.5)