FastQCFastQC Report
Fri 10 Feb 2017
SRR3558922.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558922.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801590
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT599372.139392273673164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298321.0648239035690448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212530.7586049350547368No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA178400.6367812563579967No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT125070.4464250657662256No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94720.3380937253488198No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG86460.3086104676273116No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC78710.2809476047530153No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT78640.2806977466367313No Hit
GTCTACAGCATGGTGAGAGCCCGTACACGTTCGGAGGGGGGACCAAGCTG69040.24643149068921577No Hit
ACTTATTACTGTCTACAGCATGGTGAGAGCCCGTACACGTTCGGAGGGGG69000.2462887146227678No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG64740.23108306354605776No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT64380.22979807894802595No Hit
GTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATGGTTAGAGAAT63260.22580034908748245No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG62890.22447967047283862No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA60250.21505645008727184No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT56000.19988649302717384No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54180.19339018200379068No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC51600.1841811257178959No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG49850.17793467281079672No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48390.1727233463854454No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA46640.16647689347834624No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC44070.15730353120906343No Hit
GCTGTAGACAGTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATG43880.1566253448934355No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG43850.15651826284359954No Hit
GTACATGGGGTTGTCATTGCAGTCAGGACTCAGCATGGACATGAGGGCCC40510.14459646129519307No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT40020.1428474544812053No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC39320.14034887331836565No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT39290.14024179126852965No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37050.1322463315474427No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36530.13039024268361896No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT35380.12628543077323948No Hit
GTGAGAGCCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAAAACGG35190.12560724445761157No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT34750.12403670772668376No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT34700.1238582376436238No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC33430.11932509753390039No Hit
GATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTG32740.11686221038767271No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC32310.11532736767335691No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT32270.11518459160690894No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA31860.11372113692581713No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA31680.1130786446268012No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT31560.11265031642745728No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC31310.11175796601215737No Hit
CCTAAGACCCTGATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATC30820.11000895919816961No Hit
CTATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTGGCA30710.10961632501543767No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT30150.10761746008516593No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG28910.1031914020252785No Hit
CAGTTGGACTGATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCA28730.10254890972626257No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT28500.10172794734418669No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG28390.10133531316145475No Hit
AGATTATTCTCTAACCATCAGCAGCCTGGAGTCTGACGATACAGCAACTT28350.10119253709500677No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28310.10104976102855878No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG4550.083.690532
TCACGCA4700.082.2854844
TATCACG4600.081.48742
CGCATAG8950.078.024161
ATAACGC7650.072.3317953
TAACGCA7700.071.862114
AGTGTAC9400.067.727283
GCATAGT10850.065.2566762
GTACAAC5950.064.199151
GTATCAC6600.063.3024331
ATAGTAC11600.061.037494
ATCACGC6150.060.949923
AGATTAC13400.059.0547182
TAGTACT12300.059.012895
CATAGTA12450.056.8702743
ATCTACG4900.055.8559532
GTATAAC10100.053.775731
TATCTAC5450.051.4715961
GAGTTCT13000.050.502821
ACAACGC7750.049.902163
Produced by FastQC (version 0.11.5)