FastQCFastQC Report
Fri 10 Feb 2017
SRR3558901.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558901.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159208
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28321.7788050851715993No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT24411.5332144113361137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13850.8699311592382292No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT9550.5998442289332194RNA PCR Primer, Index 26 (100% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.5332646600673333No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT6170.3875433395306768RNA PCR Primer, Index 26 (100% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.2788804582684287No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG3710.23302849103060147RNA PCR Primer, Index 26 (100% over 29bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT3690.23177227275011306No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC2750.17273001356715742No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.15137430279885433No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT2340.14697753881714487No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2330.14634942967690065No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT2190.13755590171348173No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC2170.1362996834329933No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2170.1362996834329933No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC2030.1275061554695744No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.12059695492688809No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT1770.11117531782322496RNA PCR Primer, Index 26 (100% over 27bp)
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG1740.10929099040249235No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1730.10866288126224813No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG1690.10615044470127129No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.10489422642078287No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC1670.10489422642078287RNA PCR Primer, Index 26 (95% over 22bp)
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT1630.10238178985980603No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA1620.10175368071956184No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATA300.00423207159.492152
TAGTCCC300.00423207159.492154
GTATCAA12300.048.8121831
TACAACG558.5117697E-443.2670142
GTACTGG1655.456968E-1240.025991
ATATGTA758.163224E-539.661434
TATCAAC15700.038.650952
ATCAACG16150.037.573993
CAACGCA16850.036.719195
TCAACGC16750.036.583234
AACGCAG18000.034.703756
AGATTAC700.002783611233.9955142
GGTCTTC700.002783611233.9955147
TATGTAG700.002783611233.9955145
TACTGGT1805.9480953E-1033.0511932
TGGTTCA1659.325959E-932.450265
TTCACTA1857.9853635E-1032.157928
ATGTAGG750.003902981831.7291456
GATATGT750.003902981831.7291453
GGGGGTT750.003902981831.7291457
Produced by FastQC (version 0.11.5)