FastQCFastQC Report
Fri 10 Feb 2017
SRR3558895.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558895.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193997
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT125016.443914081145586No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT37581.9371433578869774RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT20271.0448615184770897RNA PCR Primer, Index 26 (100% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19350.9974381047129595No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG13060.6732062866951551RNA PCR Primer, Index 26 (100% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9380.48351263163863356No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC6740.34742805301112906RNA PCR Primer, Index 26 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6140.31649973968669615No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT5390.2778393480311551RNA PCR Primer, Index 26 (100% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGT5090.26237519136893867No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG5050.2603133038139765No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT4630.23866348448687352RNA PCR Primer, Index 26 (100% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT4540.23402423748820855No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGT4000.206188755496219No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG3610.18608535183533767RNA PCR Primer, Index 26 (96% over 27bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA3600.1855698799465971RNA PCR Primer, Index 26 (100% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.17474497028304561No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3020.15567251039964536No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2980.1536106228446832No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC2660.13711552240498565No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA902.2139559E-433.478571
GTGTTAT750.003666872432.1394271
CATGTCA750.003907473831.7251519
CGTATTA953.2763294E-431.3157882
CACGTAA400.00536463629.74232974-75
GTATCAA10700.028.7227341
CATGGGT850.007204892627.9927854
TCTTAGA900.00950981126.4444432
TAACACG450.00953330426.43762872-73
TACCGAG900.00952177726.4376287
CTACACC1401.0533387E-425.4934254
CTTATAC71900.024.8085981
TATACAC72700.024.5466293
TTATACA73150.024.4019132
ATCAACG13650.023.5323943
TACACAT77000.023.2530945
TCAACGC14100.023.2032384
CAACGCA13900.023.1091485
ATACACA77500.023.1030754
GTATACC1300.002049122222.8787163
Produced by FastQC (version 0.11.5)