FastQCFastQC Report
Fri 10 Feb 2017
SRR3558894.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558894.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205884
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT92984.516135299489033No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT29211.4187600784908005RNA PCR Primer, Index 17 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26091.2672184336811019No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT15620.7586796448485555RNA PCR Primer, Index 17 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13310.6464805424413748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8600.4177109440267335No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG8560.41576810242660917RNA PCR Primer, Index 17 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC5880.28559771521827826No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT4470.2171125488138952RNA PCR Primer, Index 17 (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.21176973441355326No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4170.20254123681296266No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGT4150.20156981601290044No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAGCTCGTATGCCGT3830.18602708321190572No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT3560.17291290241106644No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA3500.16999864001087991TruSeq Adapter, Index 25 (96% over 27bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3480.16902721921081776No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG3270.15882730081016494No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.1398845952089526No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG2830.1374560432087972TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAACCTCGTATGCCGT2660.12919896640826875No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC2490.12094188960774029No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2170.10539915680674555No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.10151347360649685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAC453.9795723E-666.104643
TAGCACC505.324916E-447.5953374
ATAGTAC558.5161766E-443.268494
GTATATA705.0903636E-542.9760131
GTGTACT705.4394746E-542.495844
CGCATAG757.646203E-540.1109471
TAAGGCA758.169945E-539.662784
GTATCAA16250.039.617271
ACTGCGT801.1947435E-437.183868
TGTACTT801.1947435E-437.183865
CATAGTA650.001936140436.6117973
TAGTACT650.001936140436.6117975
CGTTAGA350.002788256933.9966718-19
GTCTTAG350.002788256933.99667106-107
TATCAAC19400.033.4433822
ATCAACG19600.033.0860443
CAGTGTA902.3807745E-433.0683822
TCAACGC20150.032.7734644
CAACGCA20600.032.3463485
AACGCAG21750.032.003766
Produced by FastQC (version 0.11.5)