FastQCFastQC Report
Fri 10 Feb 2017
SRR3558893.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558893.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164853
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT53653.2544145390135455No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28661.7385185589585874No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT21121.2811413805026297TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14380.8722922846414685No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT11360.6890987728461114RNA PCR Primer, Index 11 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9200.5580729498401606No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG6620.40156988347194167TruSeq Adapter, Index 11 (96% over 28bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4660.28267608111469006No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.2741836666606007No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3970.240820609876678No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT3370.20442454793058057TruSeq Adapter, Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC3190.19350572934675137No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.17106149114665795No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGT2660.16135587462769863No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT2590.1571096674006539No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.1571096674006539No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGT2550.15468326327091408No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2160.13102582300595075No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG2090.12677961577890606TruSeq Adapter, Index 11 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGT2050.12435321164916624No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.11707399925994673No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC1750.10615518067611752No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1690.10251557448150778No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG1660.10069577138420291No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA250.00205435871.3943566
TAGTACT250.00205435871.3943565
ATAGTAC359.1328075E-567.994634
GCCTTAG300.00407600960.060011
GTTACTC453.1641894E-452.884713
GCATAGT453.1641894E-452.884712
CGCATAG350.007501336351.480011
AGTGCAA350.007786914250.9959685
GTATCAA17450.042.6787451
CATGGGT905.1314164E-639.6635324
CATAGTA600.001305763439.6635323
ATGGGAC957.437462E-637.575985
TACAACG801.1935768E-437.184562
CCTTATA650.001847098536.9600071
GTACAAC650.001847098536.9600071
TATCAAC20200.036.81642
TCAACGC21050.036.742944
ATCAACG20700.036.2145273
AACGCAG21600.035.8073586
CAACGCA21500.035.6971785
Produced by FastQC (version 0.11.5)