FastQCFastQC Report
Fri 10 Feb 2017
SRR3558878.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558878.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14160315
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3896802.751916182655541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1787091.262041133971949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1323660.9347673409807621No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471280.33281745497893234No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257960.18217108870812548No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT230980.1631178402457855No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164340.11605674026319329No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161130.11378984153954201No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC156510.11052720225503457No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145710.10290025327826394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA340600.091.833051
GTATCAA1177450.083.448921
TATCAAC1624950.061.1987272
ATCAACG1688100.058.862063
TCAACGC1730100.057.5498164
CAACGCA1744800.057.034275
AACGCAG1785650.055.8327756
ACGCAGA1999750.049.8134887
CGCAGAG2025250.049.201848
GCAGAGT2125950.046.8293239
AGAGTAC2083250.036.02247610-11
CGTATCA20800.033.2627641
AGTACTT1551200.032.57533612-13
CAGAGTA2103400.031.72391910-11
AGTACAT791600.031.015212-13
TACTGGT118350.030.4114512
GAGTACT1484000.030.30269212-13
GTACTGG134200.030.0362821
TACTTTT1726100.029.19167114-15
GGTTCAC119900.029.0216666
Produced by FastQC (version 0.11.5)