FastQCFastQC Report
Fri 10 Feb 2017
SRR3558860.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558860.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930072
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450522.334213438669645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220291.1413563846322832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164610.8528697375020206No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA111890.5797193058082808No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103170.5345396441169035No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87930.4555788592342669No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT67490.3496760742604421No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG55460.2873467932802507No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54240.28102578556654884No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT52180.2703526086073473No Hit
ACTTATTACTGTCTACAGCATGGTGAGAGCCCTCCGACGTTCGGTGGAGG48810.2528921200867118No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG46220.23947293157975452No Hit
GTCTACAGCATGGTGAGAGCCCTCCGACGTTCGGTGGAGGCACCAAGCTG45810.2373486584956416No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT45150.23392909694560618No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG40360.20911136993853077No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC40170.2081269507044297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37490.19424145834974033No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC37100.1922208083429012No Hit
GTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATGGTTAGAGAAT36460.18890486987013955No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34940.18102951599733066No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG33850.1753820582859085No Hit
GTACATGGGGTTGTCATTGCAGTCAGGACTCAGCATGGACATGAGGGCCC33030.17113351211768266No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32590.16885380441765904No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA32430.16802481979946862No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30390.15745526591754092No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT28450.1474038274219822No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA27350.14170455817192312No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT27310.14149731201737553No Hit
GCTGTAGACAGTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATG26220.13584985430595337No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG26220.13584985430595337No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC25430.13175674275363822No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT25380.1314976850604537No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT25330.13123862736726918No Hit
GATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTG24360.12621290811948985No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG23490.12170530425807949No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT23370.12108356579443669No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC23120.11978827732851416No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT22950.11890748117168687No Hit
CTATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTGGCA22770.11797487347622265No Hit
CCTAAGACCCTGATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATC22670.11745675808985363No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT21790.11289734268980639No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT21650.11217198114888978No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA21210.10989227344886615No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT21170.10968502729431856No Hit
ATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTGGCAGT20760.10756075421020563No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20310.10522923497154511No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20230.10481474266244989No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA20190.1046074965079023No Hit
GTCTCCATCCTCCATGTATGCATCGCTGGGAGAGAGAGTCACTATCACTT20180.10455568496926539No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA20110.10419300419880709No Hit
AGATTATTCTCTAACCATCAGCAGCCTGGAGTCTGACGATACAGCAACTT19930.10326039650334289No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC19750.10232778880787867No Hit
ATTTAAGCTGGTACCAGCAGAAACCATGGAAATCTCCTAAGACCCTGATC19610.10160242726696206No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC19530.10118793495786686No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7000.079.4607541
TATCACG3650.076.6115342
TACAACG3600.076.02292
GTACAAC3900.075.144861
TAACGCA5850.072.209054
ACGCATA2350.071.261991
TCACGCA4100.069.6540764
AGATTAC10150.065.0648042
ATCACGC4300.065.030723
GTATCAC5250.063.7964551
AGTGTAC5100.062.9959683
GTATAAC6400.060.7436941
ATAGTAC9300.060.135944
GCATAGT9750.057.970652
TAGTACT9800.057.0677725
TACGCAG2750.054.0874445
ATCTACG2550.053.6632352
AAACGCA6050.053.104045
ATAACGC8100.052.150983
CGCAAAG4400.050.294081
Produced by FastQC (version 0.11.5)