FastQCFastQC Report
Fri 10 Feb 2017
SRR3558830.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558830.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3870910
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT989652.5566339697900493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489221.2638371855713515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327660.8464676264754282No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184180.47580543076434223No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158550.4095936097713458No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA147390.3807631797174308No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT109950.2840417369559096No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104690.27045320092691383No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT102810.2655964618138887No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG100130.2586730252059593No Hit
TCATTATGGTACTCCTCCGACGTTCGGTGGAGGCACCAAGCTGGAAATCA88470.22855090921772916No Hit
TTATGGTACTCCTCCGACGTTCGGTGGAGGCACCAAGCTGGAAATCAAAC82310.21263733850696606No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC80010.2066955832091162No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78860.20372470556019126No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG75650.1954320818618878No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT72710.1878369685681145No Hit
GACATGAGTGTGCCCACTCAGGTCCTGGGGTTGCTGCTGCTGTGGCTTAC71980.1859511071040143No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69560.17969934718192881No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA64450.16649831693322759No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT63230.16334660325349853No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG60980.1575340165490802No Hit
CCCTATCTGCATCTGTGGGAGAAACTGTCACCATCACATGTCGAGCAAGT54920.14187878302518012No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT54260.14017375759188408No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT53970.13942457974998126No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT53850.13911457512574563No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA53350.13782288919143043No Hit
GGTATCAGCAGAAACAGGGAAAATCTCCTCAGCTCCTGGTCTATAATGCA52280.13505868129199594No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52270.13503284757330963No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT51590.13327615470264098No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG50760.13113195605167777No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC50300.1299436049921078No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG49700.12839358187092959No Hit
ATCTGCATCTGTGGGAGAAACTGTCACCATCACATGTCGAGCAAGTGAGA49210.1271277296553007No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC49060.12674022387500614No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA48170.1244410229119251No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT47800.12348517532053185No Hit
GGTCTATAATGCAAAAACCTTAGCAGAAGGTGTGCCATCAAGGTTCAGTG47180.12188348476198103No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC47150.12180598360592212No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG46450.11999762329788086No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA43980.11361669478236384No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT43780.11310002040863776No Hit
GTATCCATCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTC42740.11041331366526216No Hit
CTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTG42290.10925079632437851No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41840.10808827898349484No Hit
GTCTCCAGCCTCCCTATCTGCATCTGTGGGAGAAACTGTCACCATCACAT40790.10537573852143294No Hit
GTACCATAATGATGTTGACAGTAATAACTCCCAAAATCTTCAGGCTGCAG40450.10449739208609862No Hit
GTTATTTAGCATGGTATCAGCAGAAACAGGGAAAATCTCCTCAGCTCCTG40390.1043423897739808No Hit
GATAAAAGCCAAGGAAAGGGAGGAGGAGGAGAAGGAGGTGGGGAGGAGGT39940.10317987243309713No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT39270.10144901328111477No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC39140.10111317493819283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG5050.086.009262
TCACGCA6450.076.565324
CGCATAG8450.071.5382841
TATCACG6950.070.200922
TAACGCA8800.064.90864
GTACAAC6800.063.3721
AGATTAC15650.061.9707832
GTATCAC8850.060.865771
ATAGTAC10650.058.1028564
ACAACGC8000.055.7808343
AGTGTAC11150.054.963713
ATCTACG4150.054.4815182
ATCACGC9050.053.91123
AAACGCA9700.052.748775
GCATAGT11750.052.6634372
GACCGTT4250.051.796277
TAGTACT12250.050.0249675
GAGTTCT15700.049.1772081
GATTACT20000.049.0871353
ACGCATA2950.048.692611
Produced by FastQC (version 0.11.5)