FastQCFastQC Report
Fri 10 Feb 2017
SRR3558736.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558736.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6182317
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1252922.0266188226841164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT608270.9838867854883533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT397160.6424128688321871No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA255300.41295197253715715No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218020.35265095594418727No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT193070.31229391828338793No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163770.2649006836757158No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120220.1944578383800119No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG113790.18405720703095618No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97840.1582578182257558No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG87250.1411283180723344No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84090.13601696580747963No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT84060.1359684403112943No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT77690.12566485995460924No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG67240.10876181211671936No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC65330.10567235552625336No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC63690.10301962840145532No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC62330.10081980590772036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG13950.060.394021
TAACGCA12950.043.1869054
TATCACG11400.042.7959822
GTATCAA775100.040.6489261
TACAACG11450.037.932492
AGATTAC31350.036.817832
ATAGTAC24250.035.330094
TCACGCA14700.034.4029434
GATTACT34150.033.799093
TATCAAC936450.033.635152
GTATAAC19050.033.5612261
ATCAACG947000.033.191333
TCAACGC968450.033.0828174
AACGCAG1002250.033.041346
CAACGCA981150.032.7695475
ATAACGC21350.031.768813
TAGTACT27450.031.6447015
ATCACGC16300.031.0259673
GCATAGT28700.030.6813032
ACGCAGA1083900.030.5578297