FastQCFastQC Report
Fri 10 Feb 2017
SRR3558714.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558714.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221510
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT542222.4407722675117376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258411.1632178113085245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175420.7896430806073347No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA125030.5628153823300367No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT92140.41476293151955196No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82510.37141403819924285No Hit
ACTTATTACTGTCTACAGCATGGTGAGAGCCCATTCACGTTCGGCTCGGG69860.3144707878875179No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT66090.2975003488618102No Hit
GTCTACAGCATGGTGAGAGCCCATTCACGTTCGGCTCGGGGACAAAGTTG61850.2784142317612795No Hit
GTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATGGTTAGAGAAT58820.26477486034273984No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG58470.26319935539340356No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC54900.2471292049101737No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG53800.24217761792654546No Hit
GTACATGGGGTTGTCATTGCAGTCAGGACTCAGCATGGACATGAGGGCCC47930.21575414920482014No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC44770.20152959023366987No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG44020.19815350819937788No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43650.19648797439579382No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42250.1901859545984488No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA39610.17830214583774098No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT39000.1755562657831835No Hit
GATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTG36900.16610323608716593No Hit
GCTGTAGACAGTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATG36710.16524796197181196No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT36430.16398755801234297No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG35580.16016133170681202No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA35300.15890092774734302No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34090.15345418206535194No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT33920.15268893680424578No Hit
CTATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTGGCA33740.1518786771160157No Hit
CCTAAGACCCTGATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATC33190.14940288362420157No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT32780.14755729211212193No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC32530.1464319314340246No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT32410.1458917583085379No Hit
ATTTAAGCTGGTACCAGCAGAAACCATGGAAATCTCCTAAGACCCTGATC32120.14458633992194497No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT31480.14170541658601582No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29920.13468316595468846No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT29740.1338729062664584No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA29740.1338729062664584No Hit
GGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACATTGATG29680.13360281970371504No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT29660.13351279084946724No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC28370.12770592975048503No Hit
ATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTGGCAGT28220.12703071334362664No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG27310.12293440047535235No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC27050.12176402537013113No Hit
GTCTCCATCCTCCATGTATGCATCGCTGGGAGAGAGAGTCACTATCACTT26910.12113382339039662No Hit
AGATTATTCTCTAACCATCAGCAGCCTGGAGTCTGACGATACAGCAACTT26900.12108880896327273No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC26890.12104379453614883No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA26880.12099878010902496No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG26880.12099878010902496No Hit
TCCTCCATGTATGCATCGCTGGGAGAGAGAGTCACTATCACTTGCAAGGC26280.11829791448159134No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT26030.11717255380349402No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA25690.11564206328128165No Hit
CCATGTATGCATCGCTGGGAGAGAGAGTCACTATCACTTGCAAGGCGAGT25360.11415658718619318No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC25330.11402154390482148No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC25020.11262609666398081No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT24230.10906995692119324No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC24140.10866482707707821No Hit
CTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTG23680.10659416342937912No Hit
GCATGGACATGAGGGCCCCTGCTCAGTTTTTTGGGATCTTGTTGCTCTGG23490.10573888931402514No Hit
GTGCAAAGACTCACTTTATTGAATATTTTCTGCAAATATTAGCATGATAA23320.10497364405291897No Hit
ATATTAGCATGATAAAAGCCAAGGAAAGGGAGGAGGAGGAGAAGGAGGTG23310.10492862962579506No Hit
GATAAAAGCCAAGGAAAGGGAGGAGGAGGAGAAGGAGGTGGGGAGGAGGT23290.10483860077154729No Hit
GTATCCATCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTC23220.10452349978168002No Hit
CTACAGCATGGTGAGAGCCCATTCACGTTCGGCTCGGGGACAAAGTTGGA22720.10227277842548538No Hit
GTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACAT22620.10182263415424643No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC22560.10155254759150306No Hit
TGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACG22450.10105738889314025No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA22380.10074228790327301No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG2600.070.9410552
CGCATAG3300.066.923341
TAACGCA3350.065.713784
GTACAAC3000.063.6676061
TCACGCA3500.062.8974724
TATCACG3550.062.0129972
ATAACGC3900.057.972033
ATAGTAC3950.057.238214
GTATCAC4250.056.17731
AGATTAC5050.054.1970672
GCATAGT4350.051.9760932
AGTGTAC4300.051.1956183
GTATAAC5750.043.598471
ATCACGC5350.042.2599873
CATAGTA5500.042.1892173
GACCGTT2850.039.6650777
TTCAACG9400.039.2431034
TTAGGAC2900.038.9811973
GTACTGG13550.037.88341
TAGTACT6000.037.681825
Produced by FastQC (version 0.11.5)