FastQCFastQC Report
Fri 10 Feb 2017
SRR3558706.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558706.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5628806
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2724944.841062207509017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1272282.2603017407244095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT913561.6230085030466497No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405750.7208455931861926No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181260.3220221126825121No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165310.2936857301530733No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156870.27869143118451767No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT151440.26904462509455823No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146450.26017951231575576No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC83690.14868162093346263No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT74190.13180415171530163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC72190.12825100030095193No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT68880.12237053471020319No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT67980.12077161657374584No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG60370.10725187544214528No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58400.10375202129901083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA759550.080.585961
GGTATCA250050.079.844781
TATCAAC1027750.059.6216052
ATCAACG1068700.057.2975653
TCAACGC1087350.056.4297184
CAACGCA1103000.055.558945
AACGCAG1122700.054.6900336
ACGCAGA1245950.049.2540447
CGCAGAG1253950.048.9436848
GCAGAGT1300100.047.1102149
GACCGTT19500.045.7690667
GGACCGT26150.041.636256
TACTGGT60600.039.861122
TTAGGAC24150.038.925323
TAGGACC24800.038.6248134
GTACTGG68250.037.9659461
GGTTCAC64500.036.0669366
AGAGTAC1289300.035.88013510-11
ACTGGTT67600.034.8530433
AGTACTT978500.033.52845812-13
Produced by FastQC (version 0.11.5)