FastQCFastQC Report
Fri 10 Feb 2017
SRR3558691.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558691.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15441326
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4332462.8057564486366005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1892591.2256654642224378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1502860.9732713369305201No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT687200.4450394998460625No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289020.18717304459474532No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281020.18199214238466307No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251170.1626609010132938No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223230.14456660004458166No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT201380.1304162608832946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA361900.094.035251
GTATCAA1192500.083.53791
TATCAAC1611400.061.905052
ATCAACG1671800.059.541543
TCAACGC1698400.058.7176064
CAACGCA1716650.058.096845
AACGCAG1767400.056.5935756
ACGCAGA1978000.050.5078287
CGCAGAG2015050.049.6071788
GCAGAGT2112300.047.22759
AGAGTAC2053000.035.96482510-11
AGTACTT1549650.032.7051912-13
CAGAGTA2078850.032.1961110-11
TACTGGT114500.031.073892
AGTACAT749300.030.70258112-13
GAGTACT1497250.030.5653512-13
TACTTTT1714950.029.60615514-15
GGACCGT47750.028.6591226
GTACTGG146900.028.4605771
GTACTTT1631850.028.04381614-15
Produced by FastQC (version 0.11.5)