FastQCFastQC Report
Fri 10 Feb 2017
SRR3558685.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558685.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662506
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1067854.010695187165775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472101.7731415440941727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353471.3275838627218117No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183670.6898388210204972No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104270.3916235306136399No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86540.3250321313829903No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77920.29265661748743477No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68960.25900411116444433No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52710.19797138485321722No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC46710.17543622436907183No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT44180.16593389836492387No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38990.1464409845461381No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA37930.14245977286060577No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT33360.12529549229184836No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC31770.11932367476354983No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29450.11061007937634695No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG28130.10565234406983495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA369000.062.9841041
TACAACG5300.059.497712
CGCATAG7550.054.5320741
TATCAAC468700.049.5962332
ATCAACG482850.047.9702953
TCAACGC498150.047.2016374
CAACGCA504500.046.6429025
AACGCAG515050.046.484576
GGTATCA172800.046.4093441
ACAACGC7400.043.4172483
AGATTAC12150.042.1136052
ACGCAGA573150.041.6686367
GTACAAC8450.041.6624871
CGCAGAG576700.041.5192078
ATAGTAC9900.041.4680984
TCACGCA5800.041.03294
GCAGAGT599450.039.814459
AGTGTAC12150.036.7269823
TACTGGT19900.035.579032
TATCACG6050.035.4035952