FastQCFastQC Report
Fri 10 Feb 2017
SRR3558668.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558668.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4161692
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1325853.185843642441584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600791.4436195662725642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422771.0158608565939045No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236830.5690714257566394No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156000.37484753797253617No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121290.29144396077364687No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98680.23711509645596068No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92400.22202508018373296No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA86620.20813649832808387No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62480.15013124469566705No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC54510.1309803800954035No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT51210.12305091294598446No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47200.11341540892502378No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG46700.11221397450844513No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45070.10829729831039875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA504550.057.648891
CGCATAG11800.057.136941
TAACGCA9100.050.3445434
TACAACG8600.047.736272
TATCAAC638000.045.6581762
ATCAACG652350.044.599633
TCAACGC669250.044.1401634
AACGCAG691650.043.7601136
CAACGCA677550.043.7048235
GTATAAC13150.041.7428971
AGATTAC22200.041.5409622
ATAACGC14000.040.798693
TATCACG7800.040.4277082
ACGCAGA764000.039.522627
CGCAGAG770750.039.3868148
GCAGAGT808700.037.582659
ATAGTAC18450.037.4080164
GCATAGT18500.037.3064652
TAGTACT19450.036.7083365
ACAACGC11450.036.3743823
Produced by FastQC (version 0.11.5)