SRR3558655.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3558655.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2666852
Sequences flagged as poor quality	0
Sequence length	125
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93987	3.5242675634043437	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43173	1.6188749881883209	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32711	1.2265772528809247	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16229	0.6085452061081755	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8509	0.3190653249599153	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7051	0.2643941246083397	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6657	0.24962015139947774	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT	6649	0.24932017224802874	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	6621	0.24827024521795735	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC	6151	0.23064647007033012	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5373	0.20147349759191738	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	4997	0.18737447747381558	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA	4782	0.17931253777862438	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC	4728	0.1772876785063438	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT	4473	0.16772584305390775	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	3584	0.13439065984914048	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG	3555	0.13330323542513794	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA	3153	0.118229283064827	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT	3141	0.11777931433765354	No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA	3066	0.1149670097928194	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	2970	0.1113672599754317	No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC	2942	0.1103173329453603	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2940	0.11024233815749805	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA	2875	0.10780500755197514	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC	2840	0.10649259876438588	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	485	57.657684	4
GTATCAA	39200	56.345367	1
CGCATAG	660	49.719482	1
TATCAAC	49025	44.940426	2
TACAACG	490	44.926933	2
ATCAACG	50415	43.642365	3
CAACGCA	52040	42.988438	5
TCAACGC	51875	42.964596	4
AACGCAG	53135	42.852768	6
TACTGGT	2550	42.23179	2
ATAACGC	655	40.87637	3
GTACTGG	2975	39.708736	1
ATAGTAC	870	38.981335	4
GACCGTT	795	38.916817	7
ACGCAGA	58725	38.783768	7
CGCAGAG	59045	38.677242	8
ACTGGTT	2770	38.018467	3
GCAGAGT	60850	37.412613	9
GTATAAC	705	37.23672	1
GGTTCAC	2815	37.19935	6

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content