FastQCFastQC Report
Fri 10 Feb 2017
SRR3558637.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558637.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4545989
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1339962.9475654252572983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT644151.4169633934441987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT428810.9432710901852162No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235910.5189409829192283No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149330.3284873764542765No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114000.25077051440291653No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102160.22472557676668378No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90390.19883462102525984No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA82350.181148700535791No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT64260.1413553794344861No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC63790.14032150099791266No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT61880.1361199950109866No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59570.13103859248229593No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC50240.11051500564563618No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA47190.10380579451468096No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG46450.10217798591241642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG13600.063.561631
GTATCAA554150.058.890251
TACAACG9450.050.998062
TATCAAC709450.045.9326362
TATCACG9250.045.668532
ATCAACG720300.045.2572673
TCACGCA9450.044.7005234
TCAACGC742850.044.6909074
CAACGCA756300.044.0918245
AACGCAG776500.044.048126
ATAGTAC20450.042.47624
TAACGCA11100.042.3438384
GCATAGT21850.040.5728262
ACGCAGA857300.039.8063967
CGCAGAG866300.039.5439348
ACAACGC12200.039.5025943
GTACAAC14600.037.9748461
GCAGAGT901250.037.9048239
TAGTACT23000.037.766055
GTATCAC16600.037.3500481