FastQCFastQC Report
Fri 10 Feb 2017
SRR3558619.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558619.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4919475
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1627483.3082391921902237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT763861.552726662906103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT492331.000777521991676No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277670.5644301475259047No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188450.3830693315851793No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148450.30175984225959074No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128070.26033265744820333No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118520.24092001687171902No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA102940.2092499707794023No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT80340.1633101093104447No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC74710.15186579868786812No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT63920.1299325639422906No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58730.11938265770229546No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG57120.11610995075694051No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC56710.11527652849135325No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC55080.11196316680133551No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA53280.10830423978168402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA614000.057.621251
CGCATAG15800.056.970521
TACAACG11100.053.0643732
TAACGCA9950.047.236444
TATCAAC777050.045.480822
ATAGTAC20750.044.7280164
ATCAACG792450.044.5369073
TCAACGC819400.044.0214924
AACGCAG847400.043.7319266
CAACGCA829600.043.6949425
ACAACGC13700.043.428043
AGATTAC24550.042.168542
AGTGTAC24300.041.8678473
TAGTACT22900.041.0478445
ACGCAGA931100.039.7815327
CGCAGAG938500.039.632688
GCATAGT23400.039.410022
GTATAAC13000.038.976781
TATCACG10400.038.9015052
GCAGAGT979800.037.889249
Produced by FastQC (version 0.11.5)