FastQCFastQC Report
Fri 10 Feb 2017
SRR3558608.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558608.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155246
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT735303.411675511751327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354131.6431070977512543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226891.0527336554620679No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135300.627770565401815No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109820.5095474020135057No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77740.36070128421535175No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA76440.3546694901649278No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT59570.27639536275673404No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56920.2640997825770237No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55690.25839277743700717No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30090.139612833059428No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT27330.12680687030622026No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG26860.12462614476491315No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC26000.12063588100847886No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23940.11107780735934553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4750.060.2119751
GTATCAA291900.052.2157751
ACAACGC4750.047.5981453
TACAACG5050.043.6014672
TATCAAC363250.041.939682
TAACGCA3700.041.8091774
AGATTAC10900.041.4932862
ATCAACG371100.041.092043
TATCACG4350.041.041452
TCAACGC378900.040.8585244
CAACGCA383400.040.48765
AACGCAG391300.040.4304436
ATAGTAC7900.037.656764
GTATAAC6100.037.118381
ACGCAGA427950.036.9123347
CGCAGAG431700.036.7846458
GATTACT12350.036.613963
GCAGAGT446250.035.5719459
AGTGTAC9550.034.888693
GTACAAC7300.034.2816471
Produced by FastQC (version 0.11.5)