FastQCFastQC Report
Fri 10 Feb 2017
SRR3558607.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558607.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682605
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT538593.2009295110854894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256511.5244813845198368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167690.9966094240775464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98400.5848074860112742No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71980.42778905328345035No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50880.3023882610594881No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39150.23267492964777828No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36280.21561804463911613No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT25300.1503620873585898No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24490.14554812329691164No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC20130.11963592168096493No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19840.11791240368357399No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18460.10971083528219636No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC17400.10341107984345702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA182750.061.5428621
CGCATAG4800.053.3654631
TACAACG3850.051.0044942
TATCAAC238350.046.9351232
ACAACGC4100.046.438983
TAACGCA4300.045.6627434
ATCAACG244700.045.591493
TCAACGC251150.045.344574
TATCACG3300.045.0797272
CAACGCA255400.044.7530865
AACGCAG263750.044.5756956
TCACGCA3750.042.839964
GTATAAC5200.041.2412871
GGTATCA88700.040.6316831
ATAACGC5300.040.4150543
ACGCAGA291550.040.121227
CGCAGAG295550.039.819798
GCAGAGT308700.038.0657239
AGATTAC10250.037.1544952
GTACAAC6600.035.2008971