FastQCFastQC Report
Fri 10 Feb 2017
SRR3558606.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558606.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3320296
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT942512.838632459274715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT466561.4051759240742392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291310.8773615364413293No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162070.4881191315473078No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112310.33825297503596063No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89170.2685603934107079No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA88890.26771709510236436No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69710.20995116098082822No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT69080.2080537397870551No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68760.2070899702918053No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT47250.1423065895329814No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA46320.1395056344374116No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC43700.13161477169505367No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC40280.12131448521457122No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT37870.11405609620347103No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC35190.10598452668075377No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT33800.10179815293576236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA428400.046.8426671
TATCAAC526700.038.0633352
ATCAACG539300.037.0459673
TCAACGC546250.036.890494
CAACGCA554000.036.3636865
AACGCAG565500.035.9713866
TAACGCA4400.035.1569634
CGCATAG5100.033.880271
ACGCAGA612950.033.0993927
CGCAGAG615450.032.993948
TATCACG4750.032.572342
GCAGAGT630200.032.1745079
TACTGGT25000.030.943722
GTACTGG30850.030.9018021
AGTACTT368950.030.8167712-13
GGTTCAC26050.028.5491376
GAGTACT341200.027.99595312-13
CATGGGG93450.027.2492874
TACTTTT423150.026.9681714-15
GACCGTT7550.026.793087
Produced by FastQC (version 0.11.5)