FastQCFastQC Report
Fri 10 Feb 2017
SRR3558602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5038658
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT885071.7565589885243253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433400.8601496668358917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275140.5460580972155681No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161400.320323387695692No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111630.22154708654566355No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85620.16992619860288197No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA67220.13340853854339785No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66640.1322574383893489No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT60830.1207265902944792No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60010.11909917283530655No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT51720.10264637925415855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA361000.055.350821
TATCAAC464900.042.902892
ATCAACG468200.042.4823233
TCAACGC475450.042.185334
CAACGCA479900.041.918145
AACGCAG494600.041.3098646
ACGCAGA544400.037.520047
CGCAGAG552000.037.0250178
GCAGAGT588750.034.6734779
GGTATCA195850.030.9969391
CGCATAG8150.030.701521
TAACGCA6850.028.6636164
AGTACTT365900.028.2368112-13
AGAGTAC563800.028.2190110-11
CATGGGG144600.027.8565884
GAGTACT323800.026.63450612-13
TACTGGT36350.026.5187242
ACATGGG354200.025.952743
TACTTTT402750.025.7271214-15
GTACTGG43650.025.5226381
Produced by FastQC (version 0.11.5)