FastQCFastQC Report
Fri 10 Feb 2017
SRR3558601.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558601.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314050
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT821023.5479786521466696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390091.685745770402541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236821.0234005315356194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163760.707677016486247No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111490.481795985393574No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88540.3826192173894255No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68610.2964931613405069No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61550.2659838810743069No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT49320.21313281908342516No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC39120.16905425552602582No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA33430.14446533134547654No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC30630.13236533350619042No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30450.13158747650223634No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT29010.12536462047060348No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25000.10803569499362589No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA24350.10522676692379161No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23340.10086212484604912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA255500.066.542611
TATCAAC331100.051.2517622
ATCAACG339000.050.0376853
TCAACGC346600.049.4211464
CAACGCA350050.048.951065
AACGCAG357500.048.4635286
GACCGTT5000.045.2182047
ACGCAGA397050.043.4113247
CGCAGAG401950.042.956128
CGCATAG4800.042.1815221
GGTATCA113200.041.7694441
GCAGAGT416800.041.382839
TATAGCG957.4775726E-637.5774545
GGACCGT9950.033.4861036
TAACGCA3400.033.2486764
CATGGGT29050.032.155354
TCACGCA4150.031.5409154
AGAGTAC412900.031.30506310-11
TACAACG4850.030.670212
GTACTGG24150.029.836841
Produced by FastQC (version 0.11.5)