FastQCFastQC Report
Fri 10 Feb 2017
SRR3558599.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558599.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600106
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT537323.3580275306760927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260541.6282671273028162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170711.0668668200731701No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104610.6537691877913089No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83930.52452775003656No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57510.35941368884311414No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43140.2696071385270726No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38850.24279641473752364No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT29020.18136298470226347No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26990.16867632519345593No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC22300.13936576701793504No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19750.123429322807364No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC18250.11405494385996928No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.11386745628102139No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA17100.10686792000029997No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT16840.10524302764941822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA186550.060.8301731
TACAACG3300.057.7109832
CGCATAG5250.053.3563231
TATCAAC238450.047.396922
ATCAACG243400.046.395513
TCAACGC251450.045.8334
CAACGCA255700.045.141015
AACGCAG262050.044.8418246
ACAACGC4450.042.7848783
GGTATCA90800.041.8776361
ACGCAGA290800.040.4494487
CGCAGAG294400.040.055878
ATAGTAC6950.039.3797844
GCAGAGT305700.038.4584549
TAACGCA3900.038.139564
GTACAAC5250.037.462951
TATCACG2400.034.7167662
TAGTACT8650.033.703915
TACTGGT12050.033.0910222
AGATTAC8550.032.715552
Produced by FastQC (version 0.11.5)