FastQCFastQC Report
Fri 10 Feb 2017
SRR3558595.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558595.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4297704
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT677991.5775632756467175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339770.7905849262769145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214400.4988710250868836No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126300.2938778473342976No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82580.19214911031564763No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68220.1587359203891194No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51070.11883089202979079No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46110.10728984592703454No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43190.10049552039879897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA265600.057.5040051
ATCAACG341700.044.2453163
TATCAAC343800.044.045822
TCAACGC348400.043.7701534
CAACGCA354450.043.1573455
AACGCAG367450.042.3267566
ACGCAGA403550.038.437167
CGCAGAG411200.037.8667688
GGTATCA136200.035.9503251
GCAGAGT435550.035.84549
TAACGCA4950.030.0498584
AGAGTAC418050.029.12635210-11
AGTACTT279450.028.00862712-13
ACATGGG270750.027.3814983
GTACATG290600.026.9344351
CATGGGG121450.026.4547624
TACATGG295800.026.4515042
GAGTACT251350.026.22804812-13
GTACTGG33950.025.4411281
CGCATAG6600.025.2709881
Produced by FastQC (version 0.11.5)