FastQCFastQC Report
Fri 10 Feb 2017
SRR3558594.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558594.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3101375
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT578941.8667204062714118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285480.9204949417597034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183610.5920277296360485No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109720.3537785659586474No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85990.2772641167224215No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61760.1991374793438394No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43740.14103421869332153No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41330.13326347184716456No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36640.1181411470718633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA223350.058.7983671
CGCATAG6600.046.0432361
TATCAAC288700.045.3263172
ATCAACG291750.044.7221573
TCAACGC295950.044.6302764
CAACGCA299150.044.3318675
AACGCAG307200.044.0610856
ACGCAGA341650.039.4440737
CGCAGAG346800.038.978428
GCAGAGT370400.036.494919
GGTATCA112950.035.1341
TAACGCA5000.032.1283724
ACAACGC7850.030.3169353
TATCACG6500.030.211522
AGAGTAC358500.029.292110-11
AGTACTT252250.028.23328812-13
CATGGGG88100.027.486124
ATAGTAC11050.027.4601464
ACATGGG211350.027.4471553
GTACATG225550.027.078251
Produced by FastQC (version 0.11.5)