FastQCFastQC Report
Fri 10 Feb 2017
SRR3558591.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558591.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5872104
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1245192.1205176202601317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT632781.0776035301827078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385910.6571920388330997No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225170.3834571049831543No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184160.3136184236518972No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137490.23414094845731614No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99720.16981988057432226No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98950.16850859589680292No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73400.12499778614275224No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60310.10270594662492352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA453750.058.907491
TATCAAC601150.044.491312
ATCAACG599550.044.4673163
TCAACGC610850.044.15124
CAACGCA616950.043.801085
AACGCAG635500.043.2434236
ACGCAGA700450.039.2930877
CGCAGAG712900.038.6986778
CGCATAG9800.037.6958471
GCAGAGT752950.036.5928579
GGTATCA225700.035.560181
TATCACG8250.033.8978582
TAACGCA7950.030.6837254
AGAGTAC731050.029.79453710-11
AGTACTT499300.029.28910812-13
CATGGGG158750.027.2465824
TACAACG11500.026.9050522
GAGTACT460200.026.53520212-13
CATGGGT66050.026.2126854
ACATGGG415900.025.792783
Produced by FastQC (version 0.11.5)