FastQCFastQC Report
Fri 10 Feb 2017
SRR3558590.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558590.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5458358
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1237782.267678301789659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT623211.1417536189454778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384760.7049006312887502No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232130.4252744140270755No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187470.3434549364479208No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA166960.30587953373523685No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT143250.2624415620961469No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134970.24727216499907118No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98300.18009078920803656No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95550.17505264403690635No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT72440.13271390407151748No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG70230.1286650674067183No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC68140.12483607707665931No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG59340.10871401252904261No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC59220.1084941661943024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10300.048.013481
GTATCAA608300.045.27251
TACAACG9900.039.0703282
TATCAAC750000.036.616712
ATCAACG758950.036.124723
TCAACGC772850.035.9831124
CAACGCA781650.035.6541255
AACGCAG804450.035.242376
TAACGCA8900.032.757544
ACGCAGA869250.032.6014677
CGCAGAG880900.032.1635558
GCAGAGT909300.031.1524529
TATCACG9650.030.2160472
GTATAAC13500.029.1294611
AGTACTT531350.028.65996712-13
CATGGGG152750.028.4345824
GACCGTT11400.027.6612827
CATGGGT59150.027.1590294
GGACCGT20650.026.795646
AGATTAC22850.026.563352
Produced by FastQC (version 0.11.5)