FastQCFastQC Report
Fri 10 Feb 2017
SRR3558587.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558587.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3490293
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800972.294850317724042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT404041.157610550174441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250090.7165301022005889No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155900.44666737147855495No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139410.39942205425160576No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97710.2799478439202669No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65020.1862880852696321No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62000.17763551655978452No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51890.14866946700463257No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT37490.10741218573913423No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT35380.101366847998148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA310550.058.3393671
CGCATAG6700.056.019931
TAACGCA5950.045.997984
ATCAACG402100.044.715513
TATCAAC404300.044.6699032
TCAACGC413600.044.1914754
CAACGCA419350.043.7132265
AACGCAG430700.043.3486866
ACGCAGA472200.039.4759187
CGCAGAG478900.038.998188
TACAACG7300.037.496872
GCAGAGT508850.036.772979
GGTATCA158500.036.685751
ACAACGC7400.036.1808363
AGATTAC14600.031.383252
TATCACG4850.030.6731472
CATGGGT44900.030.610014
AGAGTAC492650.029.32901410-11
ATAGTAC12600.029.2764874
GTATAAC10800.029.236741