FastQCFastQC Report
Fri 10 Feb 2017
SRR3558572.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558572.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2363488
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT553072.3400584221286507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270581.1448333987733383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172030.7278649182902558No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105860.4478973449410363No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102690.4344849645947007No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65510.2771750903749035No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA49220.208251533327015No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42480.179734358710516No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42110.17816887583097524No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32330.1367893553933847No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24620.10416807701160319No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3600.061.1640662
GTATCAA243000.050.988321
CGCATAG4600.047.936511
TAACGCA5350.047.8211364
TACAACG4750.041.344472
TATCAAC303450.040.674172
TCAACGC311700.040.448224
ATCAACG304950.040.38753
CAACGCA314400.040.0819325
AACGCAG323450.039.8793566
TCACGCA5950.036.9990044
GTATAAC7850.036.4413151
ACGCAGA355300.036.220747
CGCAGAG360900.035.8070878
ACAACGC5600.035.061553
GCAGAGT374900.034.3905839
ATCACGC6600.033.355163
CATGGGT40600.033.1197934
GTATCAC8450.031.7379071
GTACAAC8600.030.4913541