FastQCFastQC Report
Fri 10 Feb 2017
SRR3558559.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558559.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665580
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464002.7858163522616746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231161.3878648879069153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157040.942854741291322No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99410.5968491456429592No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90350.5424536797992291No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55820.33513851030872127No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38650.23205129744593478No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38470.2309705928265229No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32610.19578765355011468No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT31700.1903240913075325No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27210.16336651496775897No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC24800.14889708089674467No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT20280.1217593871204025No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20020.12019836933680761No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC19860.1192377430084415No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA19750.1185773124076898No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG17790.10680963988520516No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT17340.10410787833667551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA211300.048.8311351
TACAACG3450.044.842922
TATCAAC256150.040.4662932
AACGCAG269950.039.8708766
ATCAACG261850.039.5364073
TCAACGC266600.039.3676034
CAACGCA270200.038.997235
ACAACGC3950.037.65683
CGCATAG3700.037.031781
GACCGTT3400.036.7486087
ACGCAGA297950.036.123997
CGCAGAG300200.035.9126978
GCAGAGT313600.034.207419
TCTAACG1051.48355375E-534.0017782
TATCACG2600.032.0401342
GTACTGG15800.031.6716461
GTACAAC4950.031.2907961
TACTGGT13650.030.9503352
GGACCGT6250.030.4628496
TAACGCA3300.028.8473934
Produced by FastQC (version 0.11.5)