FastQCFastQC Report
Fri 10 Feb 2017
SRR3558554.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558554.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3940662
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT765881.94353131529677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376390.9551440849278624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236860.6010665213103789No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA216220.5486895349055565No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT187070.4747171921875055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144120.36572535274530016No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137820.3497381911973166No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86200.21874497229145765No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG79500.2017427528674116No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG79110.20075307143825072No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT74640.18940979967325286No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62020.15738472368348264No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC58160.14758941517948No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56870.1443158535291786No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG53740.13637302564898995No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC53470.13568786158264778No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT49560.12576567084413734No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC48980.1242938369238468No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC44840.11378798790660047No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA44190.11213851885799898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA7400.046.6311344
CGCATAG8700.041.81031
TACAACG8050.035.4815182
TATCACG6450.033.2123532
GTATAAC11500.033.1859931
GTATCAA616350.030.9402451
CATGGGG108700.030.3768794
GTACTGG32650.029.952521
TCACGCA7500.029.3508114
ACGGTAC10250.027.8610233
TACTGGT28000.027.8400552
AACGCAG722700.027.373126
AGTACTT371600.027.3150712-13
ACAACGC11500.026.902043
TATCAAC709750.026.786892
TAGTACT14550.026.5788125
TCAACGC726550.026.5477184
ATCAACG715850.026.4874233
CAACGCA731900.026.46785
GGTTCAC27050.026.3939676
Produced by FastQC (version 0.11.5)