FastQCFastQC Report
Fri 10 Feb 2017
SRR3558553.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558553.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4114760
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT903892.1967016302287377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT444611.0805247450641107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284570.6915834702388475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170820.4151396436244155No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123680.3005764613245973No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88040.21396144611107332No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71160.172938397379191No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA63770.15497866218199846No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62330.15147906560771465No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT48600.11811138438207819No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA376050.055.0925941
TATCAAC476250.043.4809272
AACGCAG490000.043.139446
ATCAACG487250.042.4427263
TCAACGC494800.042.2159424
CAACGCA499100.041.9595155
ACGCAGA545850.038.747327
CGCAGAG554200.038.2069248
GCAGAGT583000.036.309319
TATCACG5400.035.262312
TAACGCA6050.034.418194
GGTATCA201250.031.0166451
CATGGGG119950.030.6523384
AGTACTT381800.029.92780312-13
AGAGTAC565850.029.41368710-11
CATGGGT41550.029.2101974
ACATGGG333400.028.4087963
GTACATG354050.027.9697781
TACATGG359050.027.610522
CGCATAG6950.027.450431