FastQCFastQC Report
Fri 10 Feb 2017
SRR3558547.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558547.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4953151
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT727371.4684995470560054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353560.7138082404513814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236490.4774536451644619No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT183850.37117786233450184No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG154660.3122456795684202No Hit
GTCCTGCCCAGTTCCTGTTTCTGTTAGTGCTCTGGATTCGGGAAACCAAC137810.27822693069522814No Hit
CTCTTAGATAGTGATGGAAAGACATATTTGAATTGGTTGTTACAGAGGCC129700.26185351506546034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128240.25890589646873274No Hit
GGCCAGGCCAGTCTCCAAAGCGCCTAATCTATCTGGTGTCTAAACTGGAC114500.23116597898993996No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC110360.2228076632430548No Hit
GTGCTGGGACCAAGCTGGAGCTGAAACGGGCTGATGCTGCACCAACTGTA109880.22183858315645938No Hit
CTCCTATGTTCATTTCCTCAAAATGATGAGTCCTGCCCAGTTCCTGTTTC109250.22056666554280294No Hit
CCTATGTTCATTTCCTCAAAATGATGAGTCCTGCCCAGTTCCTGTTTCTG107720.21747772276678018No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT104210.21039132463355148No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA103000.20794843524859227No Hit
GTCTGGGACTGTGCTGGCAAGGCCTGGGGCTTCAGTGAAGATGTCCTGCA101350.20461722245092065No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93390.1885466443482139No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT87340.17633219742341794No Hit
CTTAGATAGTGATGGAAAGACATATTTGAATTGGTTGTTACAGAGGCCAG80670.16286602205343628No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT80440.16240167117860935No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT78930.15935310673952804No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT78540.15856572916916928No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG77940.15735437906092506No Hit
GTCCCAGCACCGAACGTGAGCGGAAAATGTGTACCTTGCCAGCAATAATA77300.15606227227879788No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT77300.15606227227879788No Hit
CCATTGGACAACCAGCCTCCATCTCTTGCAAGTCAAGTCAGAGCCTCTTA77060.15557773223550017No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT74420.1502477917592256No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA73850.14909700915639357No Hit
GTGCAGGGCCAGAGAAGCCATCCCGTGGTGGGACGAACACATTTACATTG73670.1487336041239203No Hit
GGTTGTTACAGAGGCCAGGCCAGTCTCCAAAGCGCCTAATCTATCTGGTG72750.14687620062461249No Hit
ATCTAAGAGGCTCTGACTTGACTTGCAAGAGATGGAGGCTGGTTGTCCAA72240.1458465530326049No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT70470.14227307021328445No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG69760.1408396392518621No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA69650.140617558398684No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69030.13936582995349828No Hit
GGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATAGGGGCTATTTAT68810.13892166824714208No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT66440.13413683531957737No Hit
TCACTCTCCTATGTTCATTTCCTCAAAATGATGAGTCCTGCCCAGTTCCT66330.13391475446639928No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC65480.1321986751463866No Hit
GTCTATTACTGTACATACGGTAGTAGCTACTATGCTATGGACTACTGGGG63670.1285444356531832No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG63140.12747440972423413No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC62620.1264245729637558No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA62240.12565738456186779No Hit
TTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACT61640.12444603445362357No Hit
CTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTG59990.12111482165595194No Hit
GTAATAGACCGCAGAGTCCTCATTTGTCAGGCTGCTGAGCTCCATGTAGG59700.12052933577030055No Hit
GTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACAT59280.11968139069452961No Hit
GTGATGGAAAGACATATTTGAATTGGTTGTTACAGAGGCCAGGCCAGTCT58680.11847004058628538No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC57760.11661263708697756No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC57740.1165722587500361No Hit
TGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACG57070.1152195844624967No Hit
GTACATGGGACTGATCACTCTCCTATGTTCATTTCCTCAAAATGATGAGT56690.1144523960606087No Hit
GTGCTGGCAAGGCCTGGGGCTTCAGTGAAGATGTCCTGCAAGACTTCTGG55900.11285745175142047No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55360.11176723665400065No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54690.11041456236646127No Hit
GTATCCATCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTC54660.11035399486104906No Hit
GTGCAGCATCAGCCCGTTTCAGCTCCAGCTTGGTCCCAGCACCGAACGTG54410.10984926564928063No Hit
TCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTCAGTCGTG54250.10952623895374883No Hit
CAGTTGGACTGATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCA51970.10492310854242078No Hit
TCTCAGGACCTTTGTCTCTAACACTCATTCCTGTTGAAGCTCTTGACAAT51830.10464046018383046No Hit
GTGCAAAGACTCACTTTATTGAATATTTTCTGCAAATATTAGCATGATAA50850.10266192167369823No Hit
GGTACACATTTTCCGCTCACGTTCGGTGCTGGGACCAAGCTGGAGCTGAA50490.10193511160875167No Hit
GTATCAACGCAGAGTACATGGGACTGATCACTCTCCTATGTTCATTTCCT50280.1015111390708662No Hit
GTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTG50210.10136981489157104No Hit
GGGTGAAGTTGATGTCTTGTGAGTGGCCTCACAGGTATAGCTGTTATGTC49730.10040073480497566No Hit
AATCTATCTGGTGTCTAAACTGGACTCTGGAGTCCCTGACAGGTTCACTG49730.10040073480497566No Hit
ATGCTGCACCAACTGTATCCATCTTCCCACCATCCAGTGAGCAGTTAACA49590.10011808644638533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA335700.055.4937971
TAACGCA3850.052.5421944
TACTGGT22700.046.1339572
GTACTGG25450.045.2081
AACGCAG440250.043.3004046
ATCAACG429300.043.2536663
TATCAAC434750.042.825242
TCAACGC439100.042.6406024
CAACGCA443100.042.40385
GACCGTT4550.041.8444027
ACTGGTT24800.041.0236633
TACAACG3250.040.2784922
ACGCAGA488250.039.031347
CATGGGT25550.036.3265154
CGCATAG4450.034.8304521
CGCAGAG557000.034.2351048
CTGGTTC30350.034.109914
GTCTATA16200.033.4866831
ACGGTAC7800.032.7992483
TTAGGAC6900.032.7660833
Produced by FastQC (version 0.11.5)