FastQCFastQC Report
Fri 10 Feb 2017
SRR3558545.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558545.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479258
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326462.2069172517573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160841.0873018770221285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111470.7535534707265399No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70930.4794971533025341No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60920.4118280921921666No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40360.2728394911502929No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33440.2260592810719969No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25180.17022047540050483No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22770.1539285236246821No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20230.13675775287340003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17120.11573369892202712No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG15450.10444425516035742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA4500.052.881664
GTATAAC4150.050.2741281
CGCATAG3000.047.6886021
GTATCAA177150.046.8406221
TATCACG1950.045.7691652
TACAACG2800.042.499942
TATCAAC214950.038.282572
AACGCAG223400.038.162376
TCAACGC222950.037.41094
ATCAACG219650.037.4041753
CAACGCA225100.037.054835
ATAACGC7500.035.6951263
ACGCAGA243750.034.9763037
CGCAGAG246650.034.6133048
ACAACGC3650.034.22823
GCAGAGT256300.033.286869
TAGTACT5300.032.5532575
ATAGTAC4950.031.2482554
CATGGGG47700.031.1802254
CATGGGT22850.030.7222774