FastQCFastQC Report
Fri 10 Feb 2017
SRR3558529.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558529.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549667
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730052.863315091735509No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348251.3658646403628394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221840.8700744057949529No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133810.5248136325253455No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117130.4593933246969114No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79410.31145243672997297No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57030.22367626831268553No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54670.21442015761273925No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52050.2041443059034768No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT44030.17268921784687963No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41270.1618642748249085No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC39470.15480452937579692No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC30970.12146684253276996No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA29140.11428943465950651No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT28150.11040657466249514No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26370.1034252708294848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA317050.052.3510861
CGCATAG5150.050.936571
TAACGCA4050.045.5402874
TACAACG3900.044.247692
TATCAAC392050.042.2101362
AACGCAG404900.042.1865966
ATCAACG398900.041.4191253
TCAACGC408950.041.0122834
CAACGCA413950.040.5312735
ACGCAGA449750.037.9135177
CGCAGAG453950.037.5758448
GCAGAGT472350.036.0869149
GACCGTT4950.033.6544047
TATCACG4700.032.9179422
GTATAAC6400.032.6041071
ACAACGC5600.031.8729523
TCACGCA5100.031.4979764
AAACGCA8500.030.7980215
TACTGGT20400.030.6278362
CATGGGG76450.030.1955744
Produced by FastQC (version 0.11.5)