FastQCFastQC Report
Fri 10 Feb 2017
SRR3558525.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558525.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7317765
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1575192.152556142483395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT771131.0537780319537453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470560.6430378674363006No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274460.37505987142249037No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209940.286890874467819No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA158290.21630921463042335No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154760.21148533739468264No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT132230.1806972484084963No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126190.1724433621467757No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118530.16197568519896444No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC107650.14710775762818293No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT92990.12707431845652326No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC83590.1142288663273554No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA76000.10385684700178265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA728600.051.2366871
AACGCAG922850.041.2926566
TATCAAC904250.041.1538542
ATCAACG915400.040.588533
TCAACGC932450.040.299384
CAACGCA939450.040.0561035
ACGCAGA1012800.037.5607037
CGCAGAG1025800.037.131868
GCAGAGT1078500.035.333999
CGCATAG12750.034.6057321
CATGGGG210350.032.15884
AGTACTT714850.028.38580912-13
GGTATCA411900.028.28521
ACATGGG622200.028.0455463
AGAGTAC1057000.028.00628310-11
GGACCGT26150.027.7570256
TACAACG11550.027.3045542
TACATGG685150.027.2352622
TACTGGT50000.027.1335122
GTACATG692050.027.0704331
Produced by FastQC (version 0.11.5)