FastQCFastQC Report
Fri 10 Feb 2017
SRR3558521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4566396
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT887921.9444656135823524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420490.9208355998910299No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297730.6520021478645304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155200.3398741589647503No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88700.19424508956297262No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68990.15108194733877656No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68000.14891393562888544No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT51540.11286800356342289No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA330000.068.287841
GGTATCA140850.055.4049151
TATCAAC430800.052.2381482
ATCAACG437900.051.262143
TCAACGC442400.051.0096784
CAACGCA448250.050.3970535
AACGCAG453900.050.044996
ACGCAGA511950.044.3471457
CGCAGAG524350.043.2984128
GCAGAGT555000.040.864379
AGAGTAC534650.031.68684810-11
GTACATG338100.029.499611
AGTACTT364400.029.39709512-13
TACATGG342750.029.2178752
ACATGGG337950.028.8719623
GAGTACT338300.028.27083212-13
CAGAGTA546700.027.98480810-11
GTACTGG32650.027.7540231
CATGGGG147950.026.6614284
TACTTTT411400.026.08203514-15
Produced by FastQC (version 0.11.5)