FastQCFastQC Report
Fri 10 Feb 2017
SRR3558515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530972
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT596582.3571181348509587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326441.28978115917521No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297781.176544031304969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247660.9785173443246311No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148940.5884695682133189No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127960.5055765136872316No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA106360.42023380740679867No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT75440.29806730378684554No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG63420.2505756681622713No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT62740.2478889533349243No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53490.2113417295805722No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49810.19680186110316514No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46420.18340779747859715No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT43740.17281898021787676No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG43080.17021128641486355No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC41520.1640476465168323No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC39530.1561850545956257No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34330.1356395882688548No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT33920.13401965727001328No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC32440.12817210146931693No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG30940.12224552464428687No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC30610.12094167774278025No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT29760.11758328420859654No Hit
CAGATTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29670.11722768959909473No Hit
CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29570.11683258447742606No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC29230.11548922706375259No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28850.11398782760141163No Hit
CTGCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT26970.10655985131404061TruSeq Adapter, Index 11 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26150.10331998931635751No Hit
GCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT25790.10189761087835031TruSeq Adapter, Index 11 (95% over 23bp)
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25720.10162103729318221No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25610.10118642165934669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG11150.079.6981661
ATAGTAC14300.062.8517724
TATCACG5350.058.9656032
GCATAGT15250.058.936422
GTATAAC6850.058.728071
TAGTACT15600.057.9658855
CATAGTA15700.057.6262783
AGATTAC18000.056.5457882
GGTTTCA11350.056.075291
TACAACG7200.052.908342
GTACAAC8100.052.630181
TAACGCA8650.050.920454
ACGCATA3750.049.6354451
GATTACT20450.049.4802933
TCACGCA7000.046.7671974
GTATCAC8800.046.3967671
ACAACGC8400.045.3500023
GTTTCAA14750.043.5821232
TATCTAC5200.042.7228161
AGTGTAC12550.042.6850133
Produced by FastQC (version 0.11.5)