FastQCFastQC Report
Fri 10 Feb 2017
SRR3558503.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558503.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298308
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298932.3024582764644443No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208281.604241828595372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165771.2768156708577627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137271.057299192487453No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA76420.5886122553354057No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74710.5754412666331872No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67110.5169035390677713No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT53550.41245990935895027No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50870.39181765805956675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45960.3539992051192783No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG41840.32226559491276335No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC33350.2568727913561343No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG32330.24901641213024955No Hit
CTGCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT29710.22883630078532982TruSeq Adapter, Index 11 (95% over 21bp)
GCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT27970.21543424210587933TruSeq Adapter, Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT27810.21420186889397586No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26960.20765488620573858No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25450.19602436401839934No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25140.1936366409203363No Hit
GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACCGTAC24470.18847607809549044No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21180.16313540392572487No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21160.16298135727423693No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT19630.1511967884354098No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC17900.13787175308170327No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17070.1314788170449539No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16720.128783000643915No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT16290.12547099763692435No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC15950.12285220456162944No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT15590.12007936483484657No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA15150.11669033850211198No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14820.11414856875256102No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC14730.11345535882086531No Hit
CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.10914205257920309No Hit
CAGATTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14160.10906502925345912No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC14110.10867991262473928No Hit
GTGTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14070.1083718193217634No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13740.10583004957221244No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG13570.104520653034565No Hit
ACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAAC13260.10213292993650196No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.1001303234671588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5900.079.409991
TACAACG2900.073.898712
GTATAAC4900.067.4216161
GTACAAC3300.067.347321
TATCACG2750.064.941292
TAACGCA5300.064.0223164
ATAGTAC7750.061.4498254
AGATTAC7650.059.9186022
GCATAGT8100.059.529522
GGTTTCA6900.057.4549371
TAGTACT8500.055.9932565
TCAAACG1600.055.8089183
CATAGTA8950.053.2107433
ACAACGC4050.052.9151273
TCACGCA3400.050.7751774
AGTGTAC5950.046.022823
TTTCAAC8550.045.952613
GAGTTCT7850.045.910721
GTTTCAA9000.043.654982
GATTACT10650.043.0401233
Produced by FastQC (version 0.11.5)