FastQCFastQC Report
Fri 10 Feb 2017
SRR3558492.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558492.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012073
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297412.938622016396051No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171581.695332253701067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159331.574293553923482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143451.4173878761709877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81320.8034993523194472No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67990.6717894855410628No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62810.6206074067779696No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43050.42536457350408513No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30750.3038318382172037No Hit
GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACAGGCA29890.2953344274573079No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28300.27962409826168666No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT25580.2527485665559698No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25370.25067361741692545No Hit
CTGCACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT25180.24879628248159966RNA PCR Primer, Index 17 (95% over 21bp)
GCACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT24070.23782869417522257RNA PCR Primer, Index 17 (95% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23630.2334811816934154No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22420.22152552236844575No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT19460.1922786202181068No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18430.1821014887266037No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG18200.17982892538384088No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18010.17795159044851508No Hit
GTATCAACGCAGAGTACATGGGCCATCTCATCCCTGCACATCTCCGAGCC16780.16579831691982694No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16080.1588818197896792No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT15560.15374385049299805No Hit
TCCCTGCACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT14500.14327029769591718No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC13520.13358720171371036No Hit
TCTCATCCCTGCACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT12850.12696712588914041No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12050.11906255774040014No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11940.11797567961994837No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11820.11678999439763732No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA11700.11560430917532628No Hit
GTGTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10810.10681047710985275No Hit
CAGATTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10670.1054271776838232No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA10490.10364864985035664No Hit
CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10480.1035498427484974No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10380.10256177172990487No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.10216654332246786No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT10310.10187012201689008No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10250.10127727940573455No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10210.10088205099829754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA3000.077.3803254
GTATAAC2900.076.6444241
GGTTACG250.001980092172.087191
CGCATAG4250.066.433291
TACAACG2850.064.7446752
GTACAAC3300.058.2522741
ATAACGC4100.058.0715453
TATCACG1950.057.997092
GTGTTAT4500.057.4027631
GCATAGT4950.056.51712
ATAGTAC4950.055.314614
GGTTTCA5750.052.2370951
TAGTACT5450.051.3066865
ACAACGC3650.050.5540583
TCACGCA2500.049.9995964
AGATTAC5800.049.2606852
TGTTATC5100.049.0192152
AGTGTAC5000.048.809133
TTAGGAC1850.048.262163
GACCGTT1800.046.2753377
Produced by FastQC (version 0.11.5)