FastQCFastQC Report
Fri 10 Feb 2017
SRR3558458.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558458.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6221410
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870453.006472809218489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT831421.336385160277172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT656961.0559664127585224No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260110.4180885040529398No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT149360.2400741953994352No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134150.21562636122679585No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC102670.1650268990470006No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT84590.13596596269977385No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84050.13509799225577482No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA83520.1342460953385165No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80090.1287328756664486No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76270.1225927884514925No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT72350.11629196596912919No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC65470.10523337957151191No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA650300.075.474941
GGTATCA242150.066.0277561
TATCAAC859250.057.0964782
ATCAACG888850.055.1486133
TCAACGC901000.054.5569844
CAACGCA914150.053.73995
AACGCAG931900.052.8439836
ACGCAGA1045850.047.0750167
CGCAGAG1058600.046.5248958
GCAGAGT1098700.044.7456259
GACCGTT16000.039.4117137
GGACCGT25900.034.6836
TACTGGT54950.034.3598562
GTACTGG63550.033.973311
TTAGGAC20950.033.8318863
AGAGTAC1082350.033.5547410-11
AGTACTT765050.031.5389712-13
GAGTACT716800.030.27169412-13
CAGAGTA1091950.029.86566410-11
GGTTCAC60750.029.573326
Produced by FastQC (version 0.11.5)