FastQCFastQC Report
Fri 10 Feb 2017
SRR3558439.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558439.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4126633
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT893332.1647914898174854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT412460.9995073465462037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283460.6869038269213666No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163120.39528593892405745No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152650.3699141648893905No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA105250.2550505460504969No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98760.23932343874534032No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT71640.17360400113118857No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69270.1678608201892439No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63120.1529576291373621No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG53420.12945178308805266No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT48740.11811081819003533No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC46480.112634198388856No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC43520.10546128041916981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG7150.052.4716872
CGCATAG9300.050.9523621
GTATCAA440650.047.2885741
TATCACG7750.044.5673522
TAACGCA10050.043.8491824
ACAACGC9550.039.285563
TATCAAC541500.038.3811072
TCAACGC557600.037.924844
ATCAACG546200.037.8768583
GTATAAC13850.037.6781581
CAACGCA564550.037.5462885
AACGCAG580550.037.5055056
ACGCAGA632750.034.392617
ATCACGC10050.034.368283
CGCAGAG641100.034.1302538
GCAGAGT660600.033.0871549
AGATTAC18100.032.2431952
GTACAAC14550.032.1552431
GTATCAC15750.030.847831
TAGTACT15700.030.6929685
Produced by FastQC (version 0.11.5)