FastQCFastQC Report
Fri 10 Feb 2017
SRR3558344.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558344.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350648
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT457833.389706274321659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227271.6826737980584137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177191.311888811888812No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103360.7652623037238422No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99160.7341661187815034No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70570.5224899455668687No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53250.3942552019475096No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44250.32762051992821223No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT38320.28371566833105294No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35880.2656502656502656No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31740.2349983119213888No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT29710.21996848919925843No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27440.20316174162328007No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG26980.19975596898673822No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26730.19790500559731328No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC24970.18487422333576178No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT23630.1749530595684442No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22190.16429151044535661No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC21440.15873862027708183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21010.15555496324727094No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA19510.14444918291072137No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG19180.14200591123668047No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT17830.13201070893378586No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA16620.12305204612896921No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC16380.12127512127512127No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15250.11290876675492059No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15190.11246453554145863No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA14930.1105395336164567No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14430.10683760683760685No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC14400.10661549123087584No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC14350.10624529855299085No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG14200.10513472051933591No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA13790.10209914056067904No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC13760.10187702495394803No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT13640.10098856252702407No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT13600.10069240838471608No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT13580.1005443313135621No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA5200.074.458114
GTATAAC5150.073.1934741
TACAACG3700.069.225912
CGCATAG5600.059.8327671
ATAACGC6350.058.15943
ACAACGC4950.052.9479833
GGTTTCA8850.052.057891
GTACAAC5000.050.2595251
TATCACG3300.048.7362142
TTCAACG9850.047.1693954
AGTGTAC6000.046.660413
ATAGTAC7700.046.415444
TCACGCA3350.044.45264
ATCTACG2150.044.3285452
GTACTGG14650.044.10881
GTTTCAA10300.043.3736462
TACTGGT14050.043.243992
GACCGTT3050.042.889637
CGCAAAG4000.041.882941
ACTGGTT14600.041.206953
Produced by FastQC (version 0.11.5)